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LONGEVITY PROTOCOLS

What the Leading Researchers and Biohackers Actually Take

A curated directory of publicly documented anti-aging protocols from researchers, physicians, and biohackers who have shared their supplement stacks, dietary strategies, and lifestyle interventions.

9 PROTOCOLS TRACKED — APRIL 2026

Published Protocols

Supplement stacks, dietary strategies, and lifestyle interventions from leading longevity figures

BJ
Bryan Johnson
Blueprint Protocol — Total Biological Optimization
Supplements Diet Exercise Sleep
Key compounds: Acarbose, Metformin, NMN/NR, NAC, Collagen peptides, Creatine, CoQ10, Vitamin D3 + K2, Lithium, EPA/DHA, Ashwagandha, DHEA, Lycopene, Spermidine, Taurine, Glucosamine, Hyaluronic acid, and 10+ more across morning/midday/evening stacks.
Diet: ~1,977 cal/day vegan, calorie-restricted, structured meal timing. Exercise: Daily structured resistance + cardio. Sleep: Strict 8:30pm bedtime, temperature-controlled.
DS
David Sinclair
NAD+ & Sirtuin Activation — Harvard Genetics
Supplements Fasting Exercise
Key compounds: NMN 1g/day, Resveratrol 1g (with yogurt fat), TMG 500mg, Metformin 800mg (evening), Spermidine, Fisetin 500mg, Quercetin, CoQ10, Vitamin D3 + K2, Taurine 2g, Low-dose aspirin 81mg.
Diet: Intermittent fasting, plant-forward, skips breakfast most days. Exercise: Prioritizes frequent movement, sauna, cold exposure.
PA
Peter Attia, MD
Medicine 3.0 — Lifespan + Healthspan
Supplements Exercise Sleep
Key compounds: Fish oil (EPA 2.5g/DHA 1g), Vitamin D3 5,000 IU, Magnesium 1g (multiple forms), Glycine 2g, Ashwagandha 600mg, Phosphatidylserine 400mg, B vitamins, AG1, Pendulum probiotic.
Exercise: 4×4 VO2 max protocol (Norwegian method), zone 2 training 3-4 hrs/week, stability/strength work. Focus: Centenarian Decathlon concept — functional fitness for the last decade of life.
AH
Andrew Huberman, PhD
Neuroscience-Driven Health — Stanford
Supplements Sleep Exercise
Key compounds: AG1, Omega-3 EPA, Vitamin D3 5,000–10,000 IU, NMN 1–2g, NR 500mg, Tongkat ali 400mg, Fadogia agrestis 600mg (cycled 8 weeks on/off), Apigenin 50mg, L-theanine 100–400mg, Magnesium L-threonate 145mg, Creatine 5g.
Sleep: Apigenin + L-theanine + Mg-threonate nightly stack. Exercise: Periodized schedule. Morning sunlight 10min daily.
RP
Rhonda Patrick, PhD
FoundMyFitness — Micronutrient Optimization
Supplements Diet Exercise
Key compounds: Omega-3 2g+, Vitamin D3 6,000 IU, Vitamin K2, Magnesium glycinate, Alpha lipoic acid 600mg, PQQ 20mg, Creatine 5g, Sulforaphane (broccoli sprouts), Cocoa extract, Melatonin 10mg.
Diet: Time-restricted eating, micronutrient-dense whole foods, emphasis on sulforaphane-rich crucifers. Exercise: Sauna 4×/week (20 min at 174°F), regular cold exposure.
MK
Matt Kaeberlein, PhD
Rapamycin Research — Dog Aging Project
Rapamycin Supplements
Key focus: Low-dose intermittent rapamycin (3–10mg weekly), mTOR inhibition as the most evidence-backed longevity intervention. Led the Dog Aging Project studying rapamycin in companion dogs.
Approach: Evidence-first, cautious about unproven supplements. Advocates rapamycin as the compound with the strongest preclinical lifespan data. Emphasis on translational research from mice to dogs to humans.
MH
Mark Hyman, MD
Functional Medicine — Pegan Diet & Longevity
Supplements Diet
Key compounds: Vitamin D3, NMN 900mg, Omega-3 fish oil, Quercetin + Fisetin (senolytic stack), Urolithin A, CoQ10, Multivitamin, HTB Rejuvenate, Seed probiotics.
Diet: Pegan diet (paleo + vegan hybrid) — plant-heavy, healthy fats, quality protein, low glycemic. Focus: Functional medicine approach targeting root causes of aging.
SW
Shawn Wells, MPH, RD
The ENERGY Formula — Mitochondrial Health & Smart Supplementation
Supplements Diet Exercise Sleep
Key compounds: NMN (NAD+ support), Paraxanthine (enfinity), Ergothioneine (mitochondrial), PQQ, CoQ10, Dihydroberberine (GlucoVantage), Spermidine, Quercetin, Resveratrol, Omega-3, Milk thistle, BPC-157 peptide, Glucosamine, B vitamins (methylated forms).
Diet: Ketogenic for 20+ years, intermittent fasting, high protein + healthy fat + fiber framework. Exercise: Blood flow restriction training, movement snacks hourly. Sleep: Blue light blocking, temperature optimization.
VL
Valter Longo, PhD
Fasting-Mimicking Diet — USC Longevity Institute
Fasting Diet
Key innovation: Fasting-Mimicking Diet (FMD/ProLon) — 5-day cycle, ~800–1,100 cal/day, designed to trigger autophagy and stem cell regeneration while providing essential nutrition.
Longevity Diet: Low protein if under 65, moderate protein if over 65, plant-heavy, fish 2–3×/week, 12-hour eating window, no snacking. Minimal supplement use. FMD cycles 2–4×/year.

⚠ These protocols are documented from publicly available sources for informational purposes only. They do not constitute medical advice. Individual protocols change over time. Always consult a physician before starting any supplement or dietary regimen. Dosages and compounds listed reflect publicly stated regimens as of early 2026.